Madonna with Child between Sts. Flavian and Onuphrius

Lotto employed oil paint on panel to achieve a rich, detailed composition. The dark background accentuates the figures, while the clothing and accessories exhibit intricate textures and vibrant, expressive colors, characteristic of Lotto's work during this period.

Created in the same year as the Recanati Polyptych (1508), the painting's location before 1693 is uncertain, though it was likely painted during Lotto's transition to Rome. The first documented record places it in the Borghese Collection by 1693, where it remains.